Basic NucleofectorTM Kit for Primary Mammalian Fibroblasts
Catalog #: VAPI-1002
For transfection of mammalian fibroblasts lacking a cell type specific Optimized Protocol for the NucleofectorTM I/II/2b Device
In addition to various cell type-specific kits for primary fibroblasts, we also provide a cell group-specific Basic NucleofectorTM Kit. This kit is suited to determine the optimal transfection conditions for any mammalian fibroblast type that lacks a cell type specific Optimized Protocol for the NucleofectorTM 2b Device (or older generations I or II). In a first experiment a selection of different NucleofectorTM Programs is tested in combination with the Basic NucleofectorTM Solution for fibroblasts. The kit is available in different variants for either 10 or 25 or 4 x 25 reactions.
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
-Optimization can be performed within 1 experiment
-Detailed protocols guiding through the optimization procedure
-Optional result fine tuning with help of our Scientific Support Team
-Transfection efficiencies up to 90%
-Viabilities up to 98%
-Kit suited for fibroblasts from different mammalian species and various organs
-Already tested for macaque dermal fibroblasts bovine fibroblasts human colon myofibroblasts mouse lung fibroblasts etc.
Content & Storage
2 x 0.45 mL NucleofectorTM Solution for Primary Mammalian Fibroblasts 2 x 0.1 mL Supplement 2 30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0) 10 Aluminum cuvette (100 µL) 10 Single use pipettes