Basic NucleofectorTM Kit for Primary Mammalian Glial Cells
Catalog #: VAPI-1006
For transfection of mammalian glial cells lacking a cell type specific Optimized Protocol for the NucleofectorTM I/II/2b Device
In addition to various cell type-specific kits for primary glial cells, we also provide a cell group-specific Basic NucleofectorTM Kit. This kit is suited to determine the optimal transfection conditions for any mammalian glial cell type that lacks a cell type specific Optimized Protocol for the NucleofectorTM 2b Device (or older generations I or II). In a first experiment a selection of different NucleofectorTM Programs is tested in combination with the Basic NucleofectorTM Solution for glial cells. The kit is available in different variants for either 10 or 25 or 4 x 25 reactions.
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
-Optimization can be performed within one experiment
-Detailed protocols guide through optimization procedure
-Fine tuning of results is possible with the help of our Scientific Support Team
-Transfection efficiency up to 60%
-Viability up to 80%
-Kit suited for various glial cells from different mammalian species
-Already tested for rat and mouse astrocytes rat oligodendrocytes
Content & Storage
2 x 0.45 mL NucleofectorTM Solution for Primary Mammalian Glial Cells 2 x 0.1 mL Supplement 1 30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0) 10 Aluminum cuvette (100 µL) 10 Single use pipettes