The P4 Primary Cell 96-well ShuttleTM Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 96-well ShuttleTM Add-On.
This kit has been determined to be optimal for transfecting following cell types:
B cell (mouse)
DC (mouse), immat.
MEF (mouse)
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 1 x 96 or 10 x 96 reactions.
High transfection efficiencies for primary cells
Compatible with liquid handling systems for automated high-throughput electroporation
Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System
Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
22.5 mL P4 Primary Cell NucleofectorTM Solution
5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
10 x 96-well NucleocuvetteTM Plate (20 μl)