The SE Cell Line 384-well NucleofectorTM Kit is one of our three kits suited for high-throughput transfection of cell lines when working with the 384-well HT NucleofectorTM System.
This kit has been determined to be optimal for transfecting following cell types:
3T3-L1, diff., 3T3-L1, undiff.
A-10, A7r5,
BHK-21, BJ
C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
DU145
GH3
HCT116, HeLa, HeLa S3
IMR-90
Jurkat (DSMZ), Jurkat E6.1
KG-1a, L6
MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
NCTC clone 929
Panc-1
T/G-HA VSMC, T-47D
U2OS, U-87 MG
WI-38
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.
High transfection efficiencies in difficult-to-transfect cell lines
Cells maintain functionality post transfection
Seamless integration into automated liquid handling environments
Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System
Transfection of low cell numbers down to 2 x 104 cell lines
High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
90 mL SE Cell Line NucleofectorTM Solution
20 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
10 x 384-well NucleocuvetteTM Plate (20 μL)