The SG Cell Line 384-well NucleofectorTM Kit is one of our three kits suited for high-throughput transfection of cell lines when working with the 384-well HT NucleofectorTM System.
This kit has been determined to be optimal for transfecting following cell types:
Ba/F3
Colo-205
H4
NIH/3T3
Raji, Ramos
THP-1
WEHI
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.
High transfection efficiencies in difficult-to-transfect cell lines
Cells maintain functionality post transfection
Seamless integration into automated liquid handling environments
Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System
Transfection of low cell numbers down to 2 x 104 cell lines
High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
22.5 mL SG Cell Line NucleofectorTM Solution
5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 384-well NucleocuvetteTM Plate (20 μl)