Product Overview

The Primary Cell Optimization 96-well ShuttleTM Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 96-well ShuttleTM Add-On.

With the unique capability of the 96-well ShuttleTM Add-On to address individual wells of a 96-well NucleocuvetteTM Plate with different programs, primary cell optimizations are easily performed within one experiment.

Complete Optimization: Using one 96-well NucleocuvetteTM Plate and our pmaxGFPTM Vector as positive control, our five Primary Cell NucleofectorTM Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 32 wells of the second 96-well NucleocuvetteTM Plate can be used for optional fine tuning or performing the first experiment with the selected conditions.

Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell NucleofectorTM Solutions and NucleofectorTM Programs. Basic Protocols are available for following cell groups:

• Endothelial cells (mammalian)

• Epithelial cells (mammalian)

• Fibroblasts (mammalian)

• Glial cells (mammalian)

• Neurons (mammalian)

• Smooth muscle cells (mammalian)

• Stem cells (human)

Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.